1cez

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF A T7 RNA POLYMERASE-T7 PROMOTER COMPLEX

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
T7 RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Sequence domains:
Structure domains:
DNA (5'-D(P*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3') Chain: T
Molecule details ›
Chain: T
Length: 17 nucleotides
Theoretical weight: 5.26 KDa
Source organism: Escherichia phage T7
Expression system: Not provided
DNA (5'-D(P*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*TP*A)-3') Chain: N
Molecule details ›
Chain: N
Length: 15 nucleotides
Theoretical weight: 4.54 KDa
Source organism: Escherichia phage T7
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21212
Unit cell:
a: 220.1Å b: 73.3Å c: 80.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.27
Expression systems:
  • Escherichia coli BL21
  • Not provided