› 1ce8

X-ray diffraction
2.1Å resolution

CARBAMOYL PHOSPHATE SYNTHETASE FROM ESCHERICHIS COLI WITH COMPLEXED WITH THE ALLOSTERIC LIGAND IMP

Released:
DOI: 10.2210/pdb1ce8/pdb
PDB entry 1ce8 coloured by chain, front view.
PDB entry 1ce8 coloured by chain, side view.
PDB entry 1ce8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The binding of inosine monophosphate to Escherichia coli carbamoyl phosphate synthetase.
Thoden JB, Raushel FM, Wesenberg G, Holden HM
J Biol Chem 274 22502-7 (1999)
PMID: 10428826

Function and Biology Details

Reactions catalysed: 
(1a) ATP + HCO(3)(-) = ADP + carboxyphosphate
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero octamer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133742 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carbamoyl phosphate synthase large chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 1073 amino acids
Theoretical weight: 117.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00968 (Residues: 1-1073; Coverage: 100%)
Gene names: JW0031, b0033, carB, pyrA
Sequence domains:
Structure domains:
Carbamoyl phosphate synthase small chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 382 amino acids
Theoretical weight: 41.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6F1 (Residues: 1-382; Coverage: 100%)
Gene names: JW0030, b0032, carA, pyrA
Sequence domains:
Structure domains:

Ligands and Environments

8 bound ligands:
Ligand MN 12 x MN
Ligand K 28 x K
Ligand CL 12 x CL
Ligand PO4 4 x PO4
Ligand ADP 8 x ADP
Ligand ORN 8 x ORN
Ligand IMP 4 x IMP
Ligand NET 4 x NET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 152.1Å b: 163.9Å c: 331.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.25
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Regulation of carbamoylphosphate synthesis in Escherichia coli: an amazing metabolite at the crossroad of arginine and pyrimidine biosynthesis.
Charlier et al. (2018)
1 more

3 mentions without citation

Mutations that improve efficiency of a weak-link enzyme are rare compared to adaptive mutations elsewhere in the genome.
Morgenthaler et al. (2019)
2 more