1c9t

X-ray diffraction
3.3Å resolution

COMPLEX OF BDELLASTASIN WITH BOVINE TRYPSIN

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cationic trypsin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Bdellastasin Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 59 amino acids
Theoretical weight: 6.35 KDa
Source organism: Hirudo medicinalis
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P82107 (Residues: 1-59; Coverage: 100%)
Sequence domains: Antistasin family
Structure domains: Antistasin; domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 95.22Å b: 108.78Å c: 95.71Å
α: 90° β: 93.7° γ: 90°
R-values:
R R work R free
0.208 0.208 0.287
Expression system: Saccharomyces cerevisiae