1c7y

X-ray diffraction
3.1Å resolution

E.COLI RUVA-HOLLIDAY JUNCTION COMPLEX

Released:
Source organism: Escherichia coli
Primary publication:
Crystal structure of the holliday junction DNA in complex with a single RuvA tetramer.
Proc. Natl. Acad. Sci. U.S.A. 97 8257-62 (2000)
PMID: 10890893

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 36-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
8 distinct DNA molecules
Macromolecules (9 distinct):
Holliday junction ATP-dependent DNA helicase RuvA Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 22.11 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A809 (Residues: 1-203; Coverage: 100%)
Gene names: JW1850, b1861, ruvA
Sequence domains:
Structure domains:
DNA (5'-D(P*DAP*DAP*DGP*DTP*DTP*DGP*DGP*DGP*DAP*DTP*DTP*DGP*DT)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4.06 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(P*DCP*DTP*DGP*DTP*DGP*DTP*DGP*DTP*DAP*DAP*DGP*DC)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.69 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(P*DGP*DCP*DTP*DTP*DAP*DCP*DAP*DCP*DAP*DCP*DAP*DGP*DA)-3') Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 3.94 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(P*DGP*DGP*DTP*DTP*DAP*DGP*DGP*DGP*DTP*DGP*DAP*DA)-3') Chain: E
Molecule details ›
Chain: E
Length: 12 nucleotides
Theoretical weight: 3.78 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(P*DTP*DTP*DCP*DAP*DCP*DCP*DCP*DTP*DAP*DAP*DCP*DCP*DA)-3') Chain: F
Molecule details ›
Chain: F
Length: 13 nucleotides
Theoretical weight: 3.86 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(P*DGP*DAP*DCP*DAP*DCP*DAP*DCP*DAP*DTP*DTP*DCP*DG)-3') Chain: G
Molecule details ›
Chain: G
Length: 12 nucleotides
Theoretical weight: 3.63 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(P*DCP*DGP*DAP*DAP*DTP*DGP*DTP*DGP*DTP*DGP*DTP*DCP*DT)-3') Chain: H
Molecule details ›
Chain: H
Length: 13 nucleotides
Theoretical weight: 4 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(P*DCP*DAP*DAP*DTP*DCP*DCP*DCP*DAP*DAP*DCP*DTP*DT)-3') Chain: I
Molecule details ›
Chain: I
Length: 12 nucleotides
Theoretical weight: 3.57 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: I432
Unit cell:
a: 158.65Å b: 158.65Å c: 158.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.249 0.279
Expression systems:
  • Escherichia coli
  • Not provided