› 1bvi

X-ray diffraction
1.9Å resolution

RIBONUCLEASE T1 (WILDTYPE) COMPLEXED WITH 2'GMP

Released:
DOI: 10.2210/pdb1bvi/pdb
PDB entry 1bvi coloured by chain, front view.
PDB entry 1bvi coloured by chain, side view.
PDB entry 1bvi coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Dissection of the structural and functional role of a conserved hydration site in RNase T1.
Langhorst U, Loris R, Denisov VP, Doumen J, Roose P, Maes D, Halle B, Steyaert J
Protein Sci 8 722-30 (1999)
PMID: 10211818

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132813 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Aspergillus oryzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
Ligand 2GP 5 x 2GP
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 59.34Å b: 60.59Å c: 100.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 0.219
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein-water interactions in a dynamic world.
Mattos C. (2002)
19 other articles

2 mentions without citation

Rapid protein fold determination using unassigned NMR data.
Meiler et al. (2003)
1 more