1bra

X-ray diffraction
2.2Å resolution

RELOCATING A NEGATIVE CHARGE IN THE BINDING POCKET OF TRYPSIN

Released:
Source organism: Rattus rattus
Primary publication:
Relocating a negative charge in the binding pocket of trypsin.
J. Mol. Biol. 230 934-49 (1993)
PMID: 8478942

Function and Biology Details

Reaction catalysed:
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anionic trypsin-2 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.81 KDa
Source organism: Rattus rattus
Expression system: Not provided
UniProt:
  • Canonical: P00763 (Residues: 24-246; Coverage: 97%)
Gene names: Prss2, Try2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I23
Unit cell:
a: 124.4Å b: 124.4Å c: 124.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 not available not available
Expression system: Not provided