› 1bkx

X-ray diffraction
2.6Å resolution

A BINARY COMPLEX OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE AND ADENOSINE FURTHER DEFINES CONFORMATIONAL FLEXIBILITY

Released:
DOI: 10.2210/pdb1bkx/pdb
PDB entry 1bkx coloured by chain, front view.
PDB entry 1bkx coloured by chain, side view.
PDB entry 1bkx coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A binary complex of the catalytic subunit of cAMP-dependent protein kinase and adenosine further defines conformational flexibility.
Narayana N, Cox S, Nguyen-huu X, Ten Eyck LF, Taylor SS
Structure 5 921-35 (1997)
PMID: 9261084

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.74 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand A 1 x A
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 55.85Å b: 73.23Å c: 98.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.205 0.34
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structures of Src-family tyrosine kinases.
Sicheri et al. (1997)
5 more

25 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
24 more