1bk6

X-ray diffraction
2.8Å resolution

KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Importin subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 422 amino acids
Theoretical weight: 46.72 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02821 (Residues: 89-510; Coverage: 78%)
Gene names: KAP60, N1606, SRP1, YNL189W
Structure domains: Leucine-rich Repeat Variant
LARGE T ANTIGEN Chains: C, E
Molecule details ›
Chains: C, E
Length: 6 amino acids
Theoretical weight: 791 Da
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
LARGE T ANTIGEN Chains: D, F
Molecule details ›
Chains: D, F
Length: 5 amino acids
Theoretical weight: 490 Da
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 161.12Å b: 75.75Å c: 84.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.256 0.307
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided