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The structure of mammalian hexokinase-1.

Mulichak AM, Wilson JE, Padmanabhan K, Garavito RM
Nat Struct Biol 5 555-60 (1998)
Cited: 63 times
EuropePMC logo PMID: 9665168

Abstract

We have determined the structures of the glucose-6-phosphate (G6P)-inhibitable 100,000 Mr Type I hexokinase from rat and the G6P-sensitive 50,000 Mr hexokinase from Schistosoma mansoni at a resolution of 2.8 and 2.6 A respectively. The structures define the glucose and G6P binding sites in these enzymes, suggest the mechanisms of intradomain G6P inhibition and activity loss in the Type I hexokinase N-terminal half, and reveal the structure of the membrane targeting motif that integrates the Type I hexokinase into the outer mitochondrial membrane.

Articles - 1bg3 mentioned but not cited (5)

  1. Structural studies of ROK fructokinase YdhR from Bacillus subtilis: insights into substrate binding and fructose specificity. Nocek B, Stein AJ, Jedrzejczak R, Cuff ME, Li H, Volkart L, Joachimiak A. J Mol Biol 406 325-342 (2011)
  2. Advanced enzymology, expression profile and immune response of Clonorchis sinensis hexokinase show its application potential for prevention and control of clonorchiasis. Chen T, Yu J, Tang Z, Xie Z, Lin Z, Sun H, Wan S, Li X, Huang Y, Yu X, Xu J. PLoS Negl Trop Dis 9 e0003641 (2015)
  3. Structural basis of complex formation between mitochondrial anion channel VDAC1 and Hexokinase-II. Haloi N, Wen PC, Cheng Q, Yang M, Natarajan G, Camara AKS, Kwok WM, Tajkhorshid E. Commun Biol 4 667 (2021)
  4. Saccharomyces cerevisiae Apn1 mutation affecting stable protein expression mimics catalytic activity impairment: implications for assessing DNA repair capacity in humans. Morris LP, Degtyareva N, Sheppard C, Heyburn L, Ivanov AA, Kow YW, Doetsch PW. DNA Repair (Amst) 11 753-765 (2012)
  5. Structural Analysis of Plasmodium falciparum Hexokinase Provides Novel Information about Catalysis Due to a Plasmodium-Specific Insertion. Dillenberger M, Werner AD, Velten AS, Rahlfs S, Becker K, Fritz-Wolf K. Int J Mol Sci 24 12739 (2023)


Reviews citing this publication (6)

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  6. How the chemical features of molecules may have addressed the settlement of metabolic steps. Del-Corso A, Cappiello M, Moschini R, Balestri F, Mura U. Metabolomics 14 2 (2017)

Articles citing this publication (52)

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