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X-ray diffraction
2.6Å resolution

HALOALKANE DEHALOGENASE MUTANT WITH TRP 175 REPLACED BY TYR

Released:

Function and Biology Details

Reaction catalysed:
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 35.14 KDa
Source organism: Xanthobacter autotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P22643 (Residues: 1-310; Coverage: 100%)
Gene name: dhlA
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-21
Spacegroup: P21212
Unit cell:
a: 95.18Å b: 72.97Å c: 41.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.253
Expression system: Escherichia coli