1bdt

X-ray diffraction
2.5Å resolution

WILD TYPE GENE-REGULATING PROTEIN ARC/DNA COMPLEX

Released:
Source organism: Salmonella virus P22
Primary publication:
Origins of DNA-binding specificity: role of protein contacts with the DNA backbone.
Proc. Natl. Acad. Sci. U.S.A. 96 811-7 (1999)
PMID: 9927650

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional repressor arc Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 53 amino acids
Theoretical weight: 6.24 KDa
Source organism: Salmonella virus P22
Expression system: Escherichia coli
UniProt:
  • Canonical: P03050 (Residues: 1-53; Coverage: 100%)
Gene name: arc
Sequence domains: Arc-like DNA binding domain
Structure domains: Met repressor-like
DNA (5'-D(*TP*AP*TP*AP*GP*TP*AP*GP*AP*GP*TP*GP*CP*TP*TP*CP*TP*AP*TP*CP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 22 nucleotides
Theoretical weight: 6.76 KDa
Source organism: Salmonella virus P22
Expression system: Not provided
DNA (5'-D(*AP*AP*TP*GP*AP*TP*AP*GP*AP*AP*GP*CP*AP*CP*TP*CP*TP*AP*CP*TP*AP*T)-3') Chain: F
Molecule details ›
Chain: F
Length: 22 nucleotides
Theoretical weight: 6.74 KDa
Source organism: Salmonella virus P22
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 62.13Å b: 56.41Å c: 52.33Å
α: 90° β: 104.14° γ: 90°
R-values:
R R work R free
0.236 0.236 0.282
Expression systems:
  • Escherichia coli
  • Not provided