› 1b8p

X-ray diffraction
1.9Å resolution

MALATE DEHYDROGENASE FROM AQUASPIRILLUM ARCTICUM

Released:
DOI: 10.2210/pdb1b8p/pdb
PDB entry 1b8p coloured by chain, front view.
PDB entry 1b8p coloured by chain, side view.
PDB entry 1b8p coloured by chain, top view.
Source organism: Aquaspirillum arcticum
Primary publication:
Structural basis for cold adaptation. Sequence, biochemical properties, and crystal structure of malate dehydrogenase from a psychrophile Aquaspirillium arcticum.
Kim SY, Hwang KY, Kim SH, Sung HC, Han YS, Cho Y
J Biol Chem 274 11761-7 (1999)
PMID: 10206992

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 35.29 KDa
Source organism: Aquaspirillum arcticum
UniProt:
  • Canonical: Q9ZF99 (Residues: 1-329; Coverage: 100%)
Gene name: mdh
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 59.08Å b: 102.17Å c: 53.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.178

Quick links

Citations

16 review citations

Psychrophilic enzymes: hot topics in cold adaptation.
Feller et al. (2003)
15 more

7 mentions without citation

Psychrophilic enzymes: from folding to function and biotechnology.
Feller G. (2013)
6 more