› 1b6i

X-ray diffraction
1.9Å resolution

T4 LYSOZYME MUTANT WITH CYS 54 REPLACED BY THR, CYS 97 REPLACED BY ALA, THR 21 REPLACED BY CYS AND LYS 124 REPLACED BY CYS (C54T,C97A,T21C,K124C)

Released:
DOI: 10.2210/pdb1b6i/pdb
PDB entry 1b6i coloured by chain, front view.
PDB entry 1b6i coloured by chain, side view.
PDB entry 1b6i coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Solid-state synthesis and mechanical unfolding of polymers of T4 lysozyme.
Yang G, Cecconi C, Baase WA, Vetter IR, Breyer WA, Haack JA, Matthews BW, Dahlquist FW, Bustamante C
Proc Natl Acad Sci U S A 97 139-44 (2000)
PMID: 10618384

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.6 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand HED 1 x HED
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P3221
Unit cell:
a: 60.87Å b: 60.87Å c: 96.73Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.185 not available
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Bacteriophage T4 genome.
Miller et al. (2003)
15 more

4 mentions without citation

Solid-state synthesis and mechanical unfolding of polymers of T4 lysozyme.
Yang et al. (2000)
3 more