› 1b4k

X-ray diffraction
1.67Å resolution

High resolution crystal structure of a MG2-dependent 5-aminolevulinic acid dehydratase

Released:
DOI: 10.2210/pdb1b4k/pdb
PDB entry 1b4k coloured by chain, front view.
PDB entry 1b4k coloured by chain, side view.
PDB entry 1b4k coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
High resolution crystal structure of a Mg2+-dependent porphobilinogen synthase.
Frankenberg N, Erskine PT, Cooper JB, Shoolingin-Jordan PM, Jahn D, Heinz DW
J Mol Biol 289 591-602 (1999)
PMID: 10356331

Function and Biology Details

Reaction catalysed: 
2 5-aminolevulinate = porphobilinogen + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176810 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-aminolevulinic acid dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 37.08 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59643 (Residues: 1-337; Coverage: 100%)
Gene names: PA5243, hemB
Sequence domains: Delta-aminolevulinic acid dehydratase
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand MG 1 x MG
Ligand SHF 2 x SHF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P4212
Unit cell:
a: 128.24Å b: 128.24Å c: 86.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.208
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

Biosynthesis of heme in mammals.
Ajioka et al. (2006)
7 more

2 mentions without citation

Detecting coevolution in and among protein domains.
Yeang et al. (2007)
1 more