› 1b2w

X-ray diffraction
2.9Å resolution

COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY

Released:
DOI: 10.2210/pdb1b2w/pdb
PDB entry 1b2w coloured by chain, front view.
PDB entry 1b2w coloured by chain, side view.
PDB entry 1b2w coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Comparison of the three-dimensional structures of a humanized and a chimeric Fab of an anti-gamma-interferon antibody.
Fan ZC, Shan L, Goldsteen BZ, Guddat LW, Thakur A, Landolfi NF, Co MS, Vasquez M, Queen C, Ramsland PA, Edmundson AB
J Mol Recognit 12 19-32
PMID: 10398393

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226411 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PROTEIN (ANTIBODY (LIGHT CHAIN)) Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.43 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
PROTEIN (ANTIBODY (HEAVY CHAIN)) Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.8 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P212121
Unit cell:
a: 69.1Å b: 74.6Å c: 104.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.33
Expression system: Not provided

Quick links

Citations

2 review citations

The immunoglobulin constant region contributes to affinity and specificity.
Torres et al. (2008)
1 more

4 mentions without citation

Toward high-resolution homology modeling of antibody Fv regions and application to antibody-antigen docking.
Sivasubramanian et al. (2009)
3 more