› 1b2v

X-ray diffraction
1.9Å resolution

HEME-BINDING PROTEIN A

Released:
DOI: 10.2210/pdb1b2v/pdb
PDB entry 1b2v coloured by chain, front view.
PDB entry 1b2v coloured by chain, side view.
PDB entry 1b2v coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
The crystal structure of HasA, a hemophore secreted by Serratia marcescens.
Arnoux P, Haser R, Izadi N, Lecroisey A, Delepierre M, Wandersman C, Czjzek M
Nat Struct Biol 6 516-20 (1999)
PMID: 10360351

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemophore HasA Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 19.29 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q54450 (Residues: 1-188; Coverage: 100%)
Gene name: hasA
Sequence domains: Heme-binding protein A (HasA)
Structure domains: Haem-binding HasA

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 41.65Å b: 80.66Å c: 63.23Å
α: 90° β: 93.58° γ: 90°
R-values:
R R work R free
0.177 0.175 0.218
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Iron metabolism in pathogenic bacteria.
Ratledge et al. (2000)
19 more

10 mentions without citation

Bacterial heme-transport proteins and their heme-coordination modes.
Tong et al. (2009)
9 more