› 1b14

X-ray diffraction
2.4Å resolution

Alcohol Dehydrogenase from Drosophila Lebanonensis Binary Complex with NAD+

Released:
DOI: 10.2210/pdb1b14/pdb
PDB entry 1b14 coloured by chain, front view.
PDB entry 1b14 coloured by chain, side view.
PDB entry 1b14 coloured by chain, top view.
Source organism: Scaptodrosophila lebanonensis
Primary publication:
The catalytic reaction and inhibition mechanism of Drosophila alcohol dehydrogenase: observation of an enzyme-bound NAD-ketone adduct at 1.4 A resolution by X-ray crystallography.
Benach J, Atrian S, Gonzàlez-Duarte R, Ladenstein R
J Mol Biol 289 335-55 (1999)
PMID: 10366509

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 254 amino acids
Theoretical weight: 27.82 KDa
Source organism: Scaptodrosophila lebanonensis
UniProt:
  • Canonical: P10807 (Residues: 1-254; Coverage: 100%)
Gene name: Adh
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P21
Unit cell:
a: 65.6Å b: 55.4Å c: 70Å
α: 90° β: 107° γ: 90°
R-values:
R R work R free
0.213 0.213 0.243

Quick links

Citations

6 review citations

Medium- and short-chain dehydrogenase/reductase gene and protein families : the SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes.
Kavanagh et al. (2008)
5 more

5 mentions without citation

Retroviral DNA integration.
Hindmarsh et al. (1999)
4 more