1axk

X-ray diffraction
2.1Å resolution

ENGINEERED BACILLUS BIFUNCTIONAL ENZYME GLUXYN-1

Released:

Function and Biology Details

Reactions catalysed:
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucanase; Endo-1,4-beta-xylanase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 394 amino acids
Theoretical weight: 43.81 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P18429 (Residues: 29-213; Coverage: 100%)
  • Canonical: P23904 (Residues: 82-237; Coverage: 73%)
Gene names: BSU18840, xynA
Sequence domains: Glycosyl hydrolases family 11
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 45.27Å b: 133.7Å c: 77.95Å
α: 90° β: 99.76° γ: 90°
R-values:
R R work R free
0.177 0.176 0.224
Expression system: Escherichia coli