› 1avk

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURES OF THE COPPER-CONTAINING AMINE OXIDASE FROM ARTHROBACTER GLOBIFORMIS IN THE HOLO-AND APO-FORMS: IMPLICATIONS FOR THE BIOGENESIS OF TOPA QUINONE

Released:
DOI: 10.2210/pdb1avk/pdb
PDB entry 1avk coloured by chain, front view.
PDB entry 1avk coloured by chain, side view.
PDB entry 1avk coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Crystal structures of the copper-containing amine oxidase from Arthrobacter globiformis in the holo and apo forms: implications for the biogenesis of topaquinone.
Wilce MC, Dooley DM, Freeman HC, Guss JM, Matsunami H, McIntire WS, Ruggiero CE, Tanizawa K, Yamaguchi H
Biochemistry 36 16116-33 (1997)
PMID: 9405045

Function and Biology Details

Reaction catalysed: 
RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylethylamine oxidase Chain: A
Molecule details ›
Chain: A
Length: 638 amino acids
Theoretical weight: 70.72 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46881 (Residues: 1-638; Coverage: 100%)
Sequence domains: Copper amine oxidase, enzyme domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 158.72Å b: 64.6Å c: 93.29Å
α: 90° β: 112.33° γ: 90°
R-values:
R R work R free
0.194 0.194 0.237
Expression system: Escherichia coli BL21(DE3)

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Citations

21 review citations

Acetogenesis and the Wood-Ljungdahl pathway of CO(2) fixation.
Ragsdale et al. (2008)
20 more

7 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
6 more