X-ray diffraction
2.8Å resolution

Atomic structure of the actin:DNASE I complex

Primary publication:
Atomic structure of the actin:DNase I complex.
Nature 347 37-44 (1990)
PMID: 2395459

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 41.52 KDa
Source organism: Oryctolagus cuniculus
  • Canonical: P68135 (Residues: 3-374; Coverage: 99%)
Gene names: ACTA, ACTA1
Structure domains:
Deoxyribonuclease-1 Chain: D
Molecule details ›
Chain: D
Length: 260 amino acids
Theoretical weight: 29.05 KDa
Source organism: Bos taurus
  • Canonical: P00639 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 132.9Å b: 56.3Å c: 109.7Å
α: 90° β: 90° γ: 90°
R R work R free
0.21 0.21 not available