› 1ak0

X-ray diffraction
1.8Å resolution

P1 NUCLEASE IN COMPLEX WITH A SUBSTRATE ANALOG

Released:
DOI: 10.2210/pdb1ak0/pdb
PDB entry 1ak0 coloured by chain, front view.
PDB entry 1ak0 coloured by chain, side view.
PDB entry 1ak0 coloured by chain, top view.
Source organism: Penicillium citrinum
Primary publication:
Recognition of single-stranded DNA by nuclease P1: high resolution crystal structures of complexes with substrate analogs.
Romier C, Dominguez R, Lahm A, Dahl O, Suck D
Proteins 32 414-24 (1998)
PMID: 9726413

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Nuclease P1 Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 29.25 KDa
Source organism: Penicillium citrinum
UniProt:
  • Canonical: P24289 (Residues: 1-270; Coverage: 100%)
Sequence domains: S1/P1 Nuclease
Structure domains: P1 Nuclease

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 2 x NAG
Ligand ZN 4 x ZN
Ligand ADS 1 x ADS
Ligand THS 3 x THS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P212121
Unit cell:
a: 41.98Å b: 74.04Å c: 102.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.235

Quick links

Citations

8 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
7 more

5 mentions without citation

A normalized root-mean-square distance for comparing protein three-dimensional structures.
Carugo et al. (2001)
4 more