1ahw

X-ray diffraction
3Å resolution

A COMPLEX OF EXTRACELLULAR DOMAIN OF TISSUE FACTOR WITH AN INHIBITORY FAB (5G9)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin kappa constant Chains: A, D
Molecule details ›
Chains: A, D
Length: 214 amino acids
Theoretical weight: 23.81 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-107; Coverage: 100%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: B, E
Molecule details ›
Chains: B, E
Length: 214 amino acids
Theoretical weight: 23.18 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: X5J519 (Residues: 1-90, 91-107; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Tissue factor Chains: C, F
Molecule details ›
Chains: C, F
Length: 219 amino acids
Theoretical weight: 24.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13726 (Residues: 33-251; Coverage: 83%)
Gene name: F3
Sequence domains: Interferon-alpha/beta receptor, fibronectin type III
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-18
Spacegroup: P2
Unit cell:
a: 142.304Å b: 73.312Å c: 115.826Å
α: 90° β: 90.89° γ: 90°
R-values:
R R work R free
0.217 0.217 0.285
Expression system: Escherichia coli BL21(DE3)