› 1a27

X-ray diffraction
1.9Å resolution

HUMAN 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE 1 C-TERMINAL DELETION MUTANT COMPLEXED WITH ESTRADIOL AND NADP+

Released:
DOI: 10.2210/pdb1a27/pdb
PDB entry 1a27 coloured by chain, front view.
PDB entry 1a27 coloured by chain, side view.
PDB entry 1a27 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human Type I 17Beta-Hydroxysteroid Dehydrogenase: Site Directed Mutagenesis and X-Ray Crystallography Structure-Function Analysis
Mazza C
Thesis, Universite Joseph Fourier (1997)

Function and Biology Details

Reactions catalysed: 
Testosterone + NAD(P)(+) = androst-4-ene-3,17-dione + NAD(P)H
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
17-beta-hydroxysteroid dehydrogenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 31.47 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P14061 (Residues: 2-290; Coverage: 88%)
Gene names: E17KSR, EDH17B1, EDH17B2, EDHB17, HSD17B1, SDR28C1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
1 bound ligand:
Ligand EST 1 x EST
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM02
Spacegroup: C2
Unit cell:
a: 111.38Å b: 48.54Å c: 60.77Å
α: 90° β: 115.13° γ: 90°
R-values:
R R work R free
0.21 0.21 0.261
Expression system: unidentified baculovirus

Quick links

Citations

2 mentions without citation

Recognition of functional sites in protein structures.
Shulman-Peleg et al. (2004)
1 more