1z9o

X-ray diffraction
1.9Å resolution

1.9 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain in Complex with the Rat ORP1 FFAT Motif

Released:
Source organism: Rattus norvegicus
Primary publication:
Structural basis of FFAT motif-mediated ER targeting.
Structure 13 1035-45 (2005)
PMID: 16004875

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dodecamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vesicle-associated membrane protein-associated protein A Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 128 amino acids
Theoretical weight: 14.55 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9Z270 (Residues: 8-132; Coverage: 50%)
Gene names: Vap33, Vapa
Sequence domains: MSP (Major sperm protein) domain
Structure domains: Immunoglobulins
Oxysterol-binding protein-related protein 1 Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 10 amino acids
Theoretical weight: 1.18 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: Q8K4M9 (Residues: 472-481; Coverage: 1%)
Gene names: Orp1, Osbpl1a

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P1
Unit cell:
a: 50.048Å b: 50.033Å c: 90.287Å
α: 90.01° β: 90° γ: 60.03°
R-values:
R R work R free
0.219 0.215 0.261
Expression systems:
  • Escherichia coli BL21
  • Not provided