› 1z2b

X-ray diffraction
4.1Å resolution

Tubulin-colchicine-vinblastine: stathmin-like domain complex

Released:
DOI: 10.2210/pdb1z2b/pdb
PDB entry 1z2b coloured by chain, front view.
PDB entry 1z2b coloured by chain, side view.
PDB entry 1z2b coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the regulation of tubulin by vinblastine.
Gigant B, Wang C, Ravelli RB, Roussi F, Steinmetz MO, Curmi PA, Sobel A, Knossow M
Nature 435 519-22 (2005)
PMID: 15917812

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-158932 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1D chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 448 amino acids
Theoretical weight: 49.95 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2HJ86 (Residues: 1-448; Coverage: 99%)
Gene name: TUBA1D
Sequence domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 49.94 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 142 amino acids
Theoretical weight: 16.71 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63043 (Residues: 48-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand GTP 2 x GTP
Ligand GDP 2 x GDP
Ligand CN2 2 x CN2
Ligand VLB 1 x VLB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P65
Unit cell:
a: 324.852Å b: 324.852Å c: 54.208Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.209 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

57 review citations

Topoisomerase I inhibitors: camptothecins and beyond.
Pommier Y. (2006)
56 more

38 mentions without citation

Interfacial inhibitors: targeting macromolecular complexes.
Pommier et al. (2011)
37 more