1z0k

X-ray diffraction
1.92Å resolution

Structure of GTP-Bound Rab4Q67L GTPase in complex with the central Rab binding domain of Rabenosyn-5

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-4A Chains: A, C
Molecule details ›
Chains: A, C
Length: 172 amino acids
Theoretical weight: 19.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20338 (Residues: 7-177; Coverage: 78%)
Gene names: RAB4, RAB4A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Rabenosyn-5 Chains: B, D
Molecule details ›
Chains: B, D
Length: 69 amino acids
Theoretical weight: 7.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H1K0 (Residues: 440-503; Coverage: 8%)
Gene names: RBSN, ZFYVE20
Sequence domains: Rabenosyn Rab binding domain
Structure domains: Rabenosyn, Rab binding domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P3121
Unit cell:
a: 81.44Å b: 81.44Å c: 137.639Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.224 0.259
Expression system: Escherichia coli