X-ray diffraction
1.81Å resolution

Phospholipase Cgamma1 SH3 in complex with a SLP-76 motif


Function and Biology Details

Reaction catalysed:
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 7.41 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P10686 (Residues: 790-851; Coverage: 5%)
Gene name: Plcg1
Sequence domains: SH3 domain
Structure domains: SH3 Domains
Lymphocyte cytosolic protein 2 Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
  • Canonical: Q13094 (Residues: 185-194; Coverage: 2%)
Gene name: LCP2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 46.723Å b: 46.723Å c: 58.112Å
α: 90° β: 90° γ: 90°
R R work R free
0.173 0.171 0.221
Expression system: Escherichia coli BL21(DE3)