PDBe 1ymr

X-ray diffraction
1.5Å resolution

The study of reductive unfolding pathways of RNase A (Y92A mutant)

Released:

Function and Biology Details

Reaction catalysed:
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.62 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P42212
Unit cell:
a: 63.988Å b: 63.988Å c: 54.838Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.261
Expression system: Escherichia coli