1xx6

X-ray diffraction
2Å resolution

X-ray structure of Clostridium acetobutylicum thymidine kinase with ADP. Northeast Structural Genomics Target CAR26.

Released:
Entry authors: Kuzin AP, Abashidze M, Forouhar F, Vorobiev SM, Acton TB, Ma L-C, Xiao R, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed:
ATP + thymidine = ADP + thymidine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 22.08 KDa
Source organism: Clostridium acetobutylicum ATCC 824
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97F65 (Residues: 1-191; Coverage: 98%)
Gene names: CA_C2887, tdk
Sequence domains: Thymidine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 53.793Å b: 67.166Å c: 103.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.266
Expression system: Escherichia coli