1xs9

Solution NMR

A MODEL OF THE TERNARY COMPLEX FORMED BETWEEN MARA, THE ALPHA-CTD OF RNA POLYMERASE AND DNA

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Multiple antibiotic resistance protein MarA Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 15.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACH5 (Residues: 1-127; Coverage: 100%)
Gene names: JW5249, b1531, marA
Sequence domains: Helix-turn-helix domain
Structure domains: Homeodomain-like
DNA-directed RNA polymerase subunit alpha Chain: D
Molecule details ›
Chain: D
Length: 84 amino acids
Theoretical weight: 9.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 249-329; Coverage: 25%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Structure domains: 5' to 3' exonuclease, C-terminal subdomain
5'-D(P*GP*AP*TP*TP*TP*AP*GP*CP*AP*AP*AP*AP*CP*GP*TP*GP*GP*CP*AP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.18 KDa
5'-D(P*AP*TP*GP*CP*CP*AP*CP*GP*TP*TP*TP*TP*GP*CP*TP*AP*AP*AP*TP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.08 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: MODEL BASED ON CHEMICAL SHIFT PERTURBATION MAPPING
Expression system: Escherichia coli