› 1xng

X-ray diffraction
1.7Å resolution

Crystal Structure of NH3-dependent NAD+ synthetase from Helicobacter pylori

Released:
DOI: 10.2210/pdb1xng/pdb
PDB entry 1xng coloured by chain, front view.
PDB entry 1xng coloured by chain, side view.
PDB entry 1xng coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Crystal structure of NH3-dependent NAD+ synthetase from Helicobacter pylori.
Kang GB, Kim YS, Im YJ, Rho SH, Lee JH, Eom SH
Proteins 58 985-8 (2005)
PMID: 15645437

Function and Biology Details

Reaction catalysed: 
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127710 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 268 amino acids
Theoretical weight: 30.38 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: O25096 (Residues: 1-260; Coverage: 100%)
Gene names: HP_0329, nadE
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand DND 2 x DND
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P31
Unit cell:
a: 63.403Å b: 63.403Å c: 125.688Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.229 0.257
Expression system: Escherichia coli

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Citations

3 review citations

Nicotinamide adenine dinucleotide metabolism as an attractive target for drug discovery.
Khan et al. (2007)
2 more

3 mentions without citation

Distinct Conformation of ATP Molecule in Solution and on Protein.
Kobayashi et al. (2013)
2 more