1x2r

X-ray diffraction
1.7Å resolution

Structural basis for the defects of human lung cancer somatic mutations in the repression activity of Keap1 on Nrf2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kelch-like ECH-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.71 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Z2X8 (Residues: 309-624; Coverage: 51%)
Gene names: Inrf2, Keap1, Kiaa0132
Sequence domains: Kelch motif
Structure domains: Kelch-type beta propeller
Nuclear factor erythroid 2-related factor 2 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q60795 (Residues: 76-84; Coverage: 2%)
Gene names: Nfe2l2, Nrf2

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P61
Unit cell:
a: 103.349Å b: 103.349Å c: 55.235Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.191 0.214
Expression systems:
  • Escherichia coli
  • Not provided