› 1x2r

X-ray diffraction
1.7Å resolution

Structural basis for the defects of human lung cancer somatic mutations in the repression activity of Keap1 on Nrf2

Released:
DOI: 10.2210/pdb1x2r/pdb
PDB entry 1x2r coloured by chain, front view.
PDB entry 1x2r coloured by chain, side view.
PDB entry 1x2r coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for defects of Keap1 activity provoked by its point mutations in lung cancer.
Padmanabhan B, Tong KI, Ohta T, Nakamura Y, Scharlock M, Ohtsuji M, Kang MI, Kobayashi A, Yokoyama S, Yamamoto M
Mol Cell 21 689-700 (2006)
PMID: 16507366

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178819 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kelch-like ECH-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.71 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Z2X8 (Residues: 309-624; Coverage: 51%)
Gene names: Inrf2, Keap1, Kiaa0132
Sequence domains: Kelch motif
Structure domains: Kelch-type beta propeller
Nuclear factor erythroid 2-related factor 2 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q60795 (Residues: 76-84; Coverage: 2%)
Gene names: Nfe2l2, Nrf2

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P61
Unit cell:
a: 103.349Å b: 103.349Å c: 55.235Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.191 0.214
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

178 review citations

Oxidative stress, inflammation, and cancer: how are they linked?
Reuter et al. (2010)
177 more

29 mentions without citation

Discovery of direct inhibitors of Keap1-Nrf2 protein-protein interaction as potential therapeutic and preventive agents.
Abed et al. (2015)
28 more