› 1woc

X-ray diffraction
2Å resolution

Crystal structure of PriB

Released:
DOI: 10.2210/pdb1woc/pdb
PDB entry 1woc coloured by chain, front view.
PDB entry 1woc coloured by chain, side view.
PDB entry 1woc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of a biologically functional form of PriB from Escherichia coli reveals a potential single-stranded DNA-binding site.
Shioi S, Ose T, Maenaka K, Shiroishi M, Abe Y, Kohda D, Katayama T, Ueda T
Biochem Biophys Res Commun 326 766-76 (2005)
PMID: 15607735

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Primosomal replication protein N Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 103 amino acids
Theoretical weight: 11.42 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07013 (Residues: 2-104; Coverage: 99%)
Gene names: JW4159, b4201, priB
Sequence domains: Single-strand binding protein family
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 103.521Å b: 70.317Å c: 74.805Å
α: 90° β: 131.59° γ: 90°
R-values:
R R work R free
0.221 0.221 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

SSB as an organizer/mobilizer of genome maintenance complexes.
Shereda et al. (2008)
2 more

7 mentions without citation

Mechanisms of bacterial DNA replication restart.
Windgassen et al. (2018)
6 more