1wa5

X-ray diffraction
2Å resolution

Crystal structure of the exportin Cse1p complexed with its cargo (Kap60p) and RanGTP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
GTP-binding nuclear protein Ran Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 20.21 KDa
Source organism: Canis lupus familiaris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62825 (Residues: 1-176; Coverage: 82%)
Gene name: RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Importin subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 530 amino acids
Theoretical weight: 59.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02821 (Residues: 1-530; Coverage: 98%)
Gene names: KAP60, N1606, SRP1, YNL189W
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Importin alpha re-exporter Chain: C
Molecule details ›
Chain: C
Length: 960 amino acids
Theoretical weight: 109.45 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33307 (Residues: 1-960; Coverage: 100%)
Gene names: CSE1, HRC135, YGL238W
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 272.672Å b: 72.261Å c: 97.64Å
α: 90° β: 92.57° γ: 90°
R-values:
R R work R free
0.234 0.234 0.268
Expression system: Escherichia coli BL21(DE3)