1w8h

X-ray diffraction
1.75Å resolution

structure of pseudomonas aeruginosa lectin II (PA-IIL)complexed with lewisA trisaccharide

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
PA-IIL domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 115 amino acids
Theoretical weight: 11.87 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HYN5 (Residues: 1-115; Coverage: 100%)
Gene names: PA3361, lecB
Sequence domains: Fucose-binding lectin II (PA-IIL)
Structure domains: Calcium-mediated lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, NDG, FUC
Carbohydrate polymer : NEW Components: NAG, GAL, NDG, FUC
Carbohydrate polymer : NEW Components: NAG, GAL, NDG, FUC
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 51.575Å b: 75.824Å c: 52.746Å
α: 90° β: 93.48° γ: 90°
R-values:
R R work R free
0.126 0.125 0.169
Expression system: Escherichia coli BL21(DE3)