1w8f

X-ray diffraction
1.05Å resolution

PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL)COMPLEXED WITH LACTO-N-NEO- FUCOPENTAOSE V(LNPFV)

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
PA-IIL domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 115 amino acids
Theoretical weight: 11.87 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HYN5 (Residues: 1-115; Coverage: 100%)
Gene names: PA3361, lecB
Sequence domains: Fucose-binding lectin II (PA-IIL)
Structure domains: Calcium-mediated lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, NAG, FUC
Carbohydrate polymer : NEW Components: BGC, GAL, NAG, FUC
Carbohydrate polymer : NEW Components: BGC, GAL, FUC
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 47.015Å b: 48.352Å c: 52.472Å
α: 85.35° β: 80.05° γ: 65.6°
R-values:
R R work R free
0.106 0.105 0.123
Expression system: Escherichia coli BL21(DE3)