1w80

X-ray diffraction
1.9Å resolution

Crystal structure of the alpha-adaptin appendage domain, from the AP2 adaptor complex, bound to 2 peptides from Synaptojanin170

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
AP-2 complex subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.05 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P17427 (Residues: 690-938; Coverage: 27%)
Gene names: Adtab, Ap2a2
Sequence domains:
Structure domains:
Synaptojanin-1 Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.47 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43426 (Residues: 1477-1488; Coverage: 1%)
Gene names: KIAA0910, SYNJ1
Synaptojanin-1 Chain: Q
Molecule details ›
Chain: Q
Length: 12 amino acids
Theoretical weight: 1.34 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43426 (Residues: 1458-1469; Coverage: 1%)
Gene names: KIAA0910, SYNJ1

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 146.599Å b: 67.322Å c: 39.721Å
α: 90° β: 94.53° γ: 90°
R-values:
R R work R free
0.179 0.177 0.224
Expression systems:
  • Escherichia coli BL21
  • Not provided