1vyj

X-ray diffraction
2.8Å resolution

Structural and biochemical studies of human PCNA complexes provide the basis for association with CDK/cyclin and rationale for inhibitor design

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 261 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
SMALL PEPTIDE SAVLQKKITDYFHPKK Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 16 amino acids
Theoretical weight: 1.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P3121
Unit cell:
a: 119.101Å b: 119.101Å c: 305.817Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.176 0.256
Expression system: Escherichia coli BL21(DE3)