1vqg

X-ray diffraction
1.82Å resolution

GENE V PROTEIN MUTANT WITH ILE 47 REPLACED BY LEU 47 (I47L)

Released:
Source organism: Enterobacteria phage f1
Primary publication:
Potential use of additivity of mutational effects in simplifying protein engineering.
Proc Natl Acad Sci U S A 93 10753-7 (1996)
PMID: 8855252

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-159614 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-Binding protein G5P Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.7 KDa
Source organism: Enterobacteria phage f1
Expression system: Escherichia coli
UniProt:
  • Canonical: P69543 (Residues: 1-87; Coverage: 100%)
Gene name: V
Sequence domains: Helix-destabilising protein
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 75.92Å b: 28.13Å c: 42.7Å
α: 90° β: 103.5° γ: 90°
R-values:
R R work R free
0.207 0.207 not available
Expression system: Escherichia coli