1vit

X-ray diffraction
3.2Å resolution

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero trimer
hetero tetramer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Thrombin light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 49 amino acids
Theoretical weight: 5.74 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00735 (Residues: 318-366; Coverage: 8%)
Gene name: F2
Sequence domains: Thrombin light chain
Thrombin heavy chain Chain: H
Molecule details ›
Chain: H
Length: 259 amino acids
Theoretical weight: 29.77 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00735 (Residues: 367-625; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hirudin-3A Chains: I, J
Molecule details ›
Chains: I, J
Length: 15 amino acids
Theoretical weight: 1.84 KDa
Source organism: Hirudo medicinalis
Expression system: Not provided
UniProt:
  • Canonical: P28507 (Residues: 51-65; Coverage: 23%)
Thrombin heavy chain Chain: F
Molecule details ›
Chain: F
Length: 150 amino acids
Theoretical weight: 17.53 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00735 (Residues: 367-516; Coverage: 25%)
Gene name: F2
Structure domains: Trypsin-like serine proteases
Thrombin heavy chain Chain: G
Molecule details ›
Chain: G
Length: 109 amino acids
Theoretical weight: 12.27 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00735 (Residues: 517-625; Coverage: 18%)
Gene name: F2
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P6122
Unit cell:
a: 116.4Å b: 116.4Å c: 200.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 not available
Expression system: Not provided