X-ray diffraction
1.9Å resolution

Crystal Structure of the heme PAS sensor domain of Ec DOS (Ferrous Form)


Function and Biology Details

Reaction catalysed:
Cyclic di-3',5'-guanylate + H(2)O = 5'-phosphoguanylyl(3'->5')guanosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Oxygen sensor protein DosP Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 19.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P76129 (Residues: 1-139; Coverage: 17%)
Gene names: JW1484, b1489, dos, dosP, pdeO, yddU
Sequence domains: PAS domain
Structure domains: Beta-Lactamase

Ligands and Environments

Cofactor: Ligand HEM 2 x HEM
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 46.4Å b: 69.18Å c: 82.45Å
α: 90° β: 90° γ: 90°
R R work R free
0.244 0.244 0.287
Expression system: Escherichia coli BL21(DE3)