1uzx

X-ray diffraction
1.85Å resolution

A complex of the Vps23 UEV with ubiquitin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.34 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P25604 (Residues: 1-161; Coverage: 42%)
Gene names: STP22, VPS23, YCL008C, YCL8C
Structure domains: Ubiquitin Conjugating Enzyme
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P0CH28 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 58.934Å b: 66.032Å c: 69.333Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.274
Expression system: Escherichia coli