1ua0

X-ray diffraction
2.1Å resolution

Aminofluorene DNA adduct at the pre-insertion site of a DNA polymerase

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 66.11 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KWC1 (Residues: 299-878; Coverage: 66%)
Gene names: GK2730, polA
Sequence domains: DNA polymerase family A
Structure domains:
DNA primer strand Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.17 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
DNA template strand with aminofluorene adduct Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.16 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 87.868Å b: 93.471Å c: 104.656Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.242
Expression systems:
  • Escherichia coli
  • Not provided