1u45

X-ray diffraction
2.01Å resolution

8oxoguanine at the pre-insertion site of the polymerase active site

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 66.11 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52026 (Residues: 297-876; Coverage: 66%)
Gene names: pol, polA
Sequence domains: DNA polymerase family A
Structure domains:
DNA primer strand Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.02 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
DNA template strand with 8-oxoguanine Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.63 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 87.281Å b: 93.141Å c: 106.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.227
Expression systems:
  • Escherichia coli
  • Not provided