1tzn

X-ray diffraction
4.3Å resolution

Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore bound to the VWA domain of CMG2, an anthrax toxin receptor

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetradecamer (preferred)
hetero 28-mer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protective antigen PA-63 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, O
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, O
Length: 562 amino acids
Theoretical weight: 63.02 KDa
Source organism: Bacillus anthracis str. A2012
Expression system: Escherichia coli
UniProt:
  • Canonical: P13423 (Residues: 203-764; Coverage: 77%)
Gene names: BXA0164, GBAA_pXO1_0164, pXO1-110, pag, pagA
Sequence domains:
Anthrax toxin receptor 2 Chains: a, b, c, d, e, f, g, h, i, j, k, l, m, o
Molecule details ›
Chains: a, b, c, d, e, f, g, h, i, j, k, l, m, o
Length: 181 amino acids
Theoretical weight: 19.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P58335 (Residues: 38-218; Coverage: 40%)
Gene names: ANTXR2, CMG2
Sequence domains: von Willebrand factor type A domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P1
Unit cell:
a: 158.8Å b: 158.846Å c: 214.084Å
α: 69.58° β: 69.07° γ: 65.58°
R-values:
R R work R free
0.325 0.322 0.33
Expression system: Escherichia coli