› 1tw5

X-ray diffraction
2.3Å resolution

beta1,4-galactosyltransferase mutant M344H-Gal-T1 in complex with Chitobiose

Released:
DOI: 10.2210/pdb1tw5/pdb
PDB 1tw5 coloured by chain and viewed from the front.
PDB 1tw5 coloured by chain and viewed from the side.
PDB 1tw5 coloured by chain and viewed from the top.
Source organism: Bos taurus
Primary publication:
Effect of the Met344His mutation on the conformational dynamics of bovine beta-1,4-galactosyltransferase: crystal structure of the Met344His mutant in complex with chitobiose.
Ramakrishnan B, Boeggeman E, Qasba PK
Biochemistry 43 12513-22 (2004)
PMID: 15449940

Function and Biology Details

Reactions catalysed: 
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine
UDP-alpha-D-galactose + D-glucose = UDP + lactose
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-1,4-galactosyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.86 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08037 (Residues: 130-402; Coverage: 68%)
Gene names: B4GALT1, GALT, GGTB2
Sequence domains:
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand MN 2 x MN
Ligand UDH 2 x UDH
Ligand SO4 5 x SO4
Ligand DIO 2 x DIO
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 50.37Å b: 92.55Å c: 143.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.257
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Glycosyltransferase engineering for carbohydrate synthesis.
McArthur et al. (2016)
4 more

1 mention without citation

Emerging structural insights into glycosyltransferase-mediated synthesis of glycans.
Moremen et al. (2019)