PDBe 1tgv

X-ray diffraction
2.2Å resolution

Structure of E. coli Uridine Phosphorylase complexed with 5-Fluorouridine and sulfate

Released:
Source organism: Escherichia coli
Entry authors: Bu W, Settembre EC, Sanders JM, Begley TP, Ealick SE

Function and Biology Details

Reaction catalysed:
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 27.47 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P12758 (Residues: 1-253; Coverage: 100%)
Gene names: JW3808, b3831, udp
Sequence domains: Phosphorylase superfamily
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: R3
Unit cell:
a: 149.972Å b: 149.972Å c: 49.566Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.175 0.224
Expression system: Escherichia coli K-12