PDBe 1t44

X-ray diffraction
2Å resolution

Structural basis of actin sequestration by thymosin-B4: Implications for arp2/3 activation

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 147 amino acids
Theoretical weight: 16.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06396 (Residues: 55-183; Coverage: 17%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 41.27 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 8-377; Coverage: 98%)
Gene names: ACTA, ACTA1
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 56.85Å b: 69.335Å c: 80.233Å
α: 90° β: 94.93° γ: 90°
R-values:
R R work R free
0.147 0.144 0.195
Expression system: Escherichia coli