1t0q

X-ray diffraction
2.15Å resolution

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
YHS domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 498 amino acids
Theoretical weight: 57.79 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O87798 (Residues: 1-498; Coverage: 100%)
Gene name: touA
Sequence domains:
Structure domains: Ribonucleotide Reductase, subunit A
Toluene, o-xylene monooxygenase oxygenase subunit Chain: B
Molecule details ›
Chain: B
Length: 330 amino acids
Theoretical weight: 38.38 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O87802 (Residues: 1-330; Coverage: 100%)
Gene name: touE
Sequence domains: Methane/Phenol/Toluene Hydroxylase
Structure domains: Ribonucleotide Reductase, subunit A
TouB protein Chain: C
Molecule details ›
Chain: C
Length: 86 amino acids
Theoretical weight: 9.99 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O87799 (Residues: 1-86; Coverage: 100%)
Gene name: touB
Sequence domains: Toluene-4-monooxygenase system protein B (TmoB)
Structure domains: TmoB-like

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3121
Unit cell:
a: 182.805Å b: 182.805Å c: 68.036Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.225
Expression system: Escherichia coli BL21(DE3)