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X-ray diffraction
2.85Å resolution

Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Replication factor C subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 516 amino acids
Theoretical weight: 56.38 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38630 (Residues: 295-785; Coverage: 57%)
Gene names: CDC44, RFC1, YOR217W, YOR50-7
Sequence domains:
Structure domains:
Replication factor C subunit 4 Chain: B
Molecule details ›
Chain: B
Length: 323 amino acids
Theoretical weight: 36.17 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40339 (Residues: 1-323; Coverage: 100%)
Gene names: O0923, RFC4, YOL094C
Sequence domains:
Structure domains:
Replication factor C subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 340 amino acids
Theoretical weight: 38.23 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38629 (Residues: 1-340; Coverage: 100%)
Gene names: N0533, RFC3, YNL290W
Sequence domains:
Structure domains:
Replication factor C subunit 2 Chain: D
Molecule details ›
Chain: D
Length: 353 amino acids
Theoretical weight: 39.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40348 (Residues: 1-353; Coverage: 100%)
Gene names: J1808, RFC2, YJR068W
Sequence domains:
Structure domains:
Replication factor C subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 354 amino acids
Theoretical weight: 39.96 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38251 (Residues: 1-354; Coverage: 100%)
Gene names: RFC5, YBR0810, YBR087W
Sequence domains: DNA polymerase III, delta subunit
Structure domains:
Proliferating cell nuclear antigen Chains: F, G, H
Molecule details ›
Chains: F, G, H
Length: 283 amino acids
Theoretical weight: 32.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15873 (Residues: 1-258; Coverage: 100%)
Gene names: POL30, YBR0811, YBR088C
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 104.209Å b: 110.481Å c: 268.206Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.251 0.306
Expression system: Escherichia coli BL21(DE3)